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KMID : 0545120040140020373
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 2 p.373 ~ p.378
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Regulation of Enzymes Involved in Methionine Biosynthesis in Corynebacterium glutamicum
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YEOM, HYE-JIN
HWANG, BYUNG-JOON/LEE, MYONG-SOK/KIM, YOUNHEE/LEE, HEUNG-SHICK
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Abstract
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The regulatory mechanism of methionhe biosynthesis in Corynebacterium glutamicum was analyzed at the protein and gene expression level. 0-Acetylhomoserine sulfhydraylase (encoded by metY) was inhibited by 10 mM methionine to a residual activity of 10% level, whereas no such inhibition was found with cystathionine y-synthase (encoded by metB) and cystathionine P-lyase (encoded by metC).The enzymatic activity of homoserine acetyltransferase (encoded by metX) was repressed to a residual activity of 25% level by 10 mM methionine which was added to the growth medium.Cystathionine y-synthase and cystathionine P-lyase were also repressed by 10mM methionine, but only to a residual activity of 50- 70% level.0-Acetylhornoserine sulfhydrylase was very sensitive to repression by 10mM methionine, showing residual activity of 13%. In addition, homoserine acetyltransferase was also repressed by 10 mM cysteine to 50% of its original activity. No repression of the enzymes by S-adenosyl methionine was observed. The pattern of repression by methionine indicated that the metB and aecD genes might be regulated by a common mechanism, while the metA and metY genes are differently regulated.
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KEYWORD
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